Paper details

Title:

Fed-Batch Operated in the Increasing Exponential Feeding Mode as an Approach to Obtain Glucose Oxidation by Glucose Oxidase and Sucrose Hydrolysis by Invertase

Author(s):

Taraboulsi-Jr, F.A., Yoriyaz, E.J., Vitolo, M.

Document(s):

Abstract:

This work is based on the use of invertase (KM = 40.2mM; Vmax = 0.064U/mL) and glucose oxidase (KM = 15.6mM; Vmax = 3.41x10­3U/mL), respectively, for hydrolyzing sucrose into glucose and fructose (inverted syrup containing both sugars at 1:1 ratio) and for oxidizing glucose into gluconic acid. Both reactions were evaluated through a fed­batch process whose sucrose (64 g/L) or glucose (32 g/L) solution was added according to an increasing exponential feeding mode. The filling­up times (.) were 1h and 4h and the exponential addition constant value (k) was set at 0.5h­1. The yield of sucrose hydrolysis by invertase was 100%, whereas the yield of glucose oxidation by glucose oxidase was over 80% either for pure glucose dissolved in water or for that present in the inverted syrup. Both enzymes were stable for 30h at pH 5.0 and 37oC.

Keywords:

biotechnology, enzymatic process, fed-batch reactor, invertase, glucose oxidase

Topic:

Biomass Conversion Technologies for Intermediates, Liquid and Gaseous Fuels, Chemicals and Materials

Subtopic:

Synthetic biofuels

Event:

22nd European Biomass Conference and Exhibition

Session:

3CV.2.54

Pages:

1220 - 1223

ISBN:

978-88-89407-52-3

Paper DOI:

10.5071/22ndEUBCE2014-3CV.2.54

Price:

FREE