Title:
Using Solid Waste of Enzymatic Hydrolysis of Sugar Cane Pretreated with Diluted Acid
Author(s):
Mori, N.R., Castro, S., Capri, M.R., Gonçalves, A.R.
Document(s):
Paper
Abstract:
Studying the use of sugarcane straw as raw material for bioethanol production, this study aimed to reuse the solid residue of the enzymatic hydrolysis of sugarcane straw pretreated with diluted acid in order to increase cellulosic ethanol production. For this purpose, sugarcane straw was submitted to the pretreatment with H2SO4 (1% w/v) at 120 ° C for 10 minutes. Then, the pretreated material was delignificated with NaOH 1% (w/v) at 100 ° C for 1 hour. The straw fresh, pre-t reated and the pulp obtained were chemically characterized to determine the content of cellulose, hemicelluloses and lignin were then subjected to enzymatic hydrolysis, which was carried out using a commercial cellulaseCelluclast 1.5 L, supplemented by ß-glycosidase (Novozym 188), both kindly donated by Novozymes (Bagsvaerd, Denmark). And to observe the structural changes in the fiber after each step was performed a Scanning Electron Microscopy (SEM). The pretreated cane straw has approximately 42% cellulose and the pulp obtained has 52% cellulose. The enzymatic conversion obtained for the pretreated straw was 60% whereas for the pulp obtained, the conversion was around 89%. The solid residue obtained after enzymatic hydrolysis were characterized chemically and passed through one after alkaline extraction using NaOH 1% (m/v) for 1 hour at 65 ° C. Then, they were again subjected to enzymatic hydrolysis. The analysis of the reuse of waste are underway.
Keywords:
bioethanol, enzymatic hydrolysis, sugar cane, waste, pretreatment
Topic:
R&D on Biomass Conversion Technologies for Heating, Electricity and Chemicals
Subtopic:
Biorefineries
Event:
21st European Biomass Conference and Exhibition
Session:
2DV.2.7
Pages:
1010 - 1011
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-2DV.2.7
Price:
FREE
