Title:
Sugar Cane Bagasse Biodelignification for Bioethanol Production
Author(s):
Bonifacino Buttiglione, S., Lopretti, M.
Document(s):
Paper
Poster
Abstract:
It was evaluated the ability to delignify sugar cane bagasse of P. ostreatus, P. chrysosporium and T. versicolor. It was compared the kinetics of production of the enzymes laccase, manganese peroxidase, lignin peroxidase and carboximethylcellulase, through solid state fermentation (SSF) and submerged fermentation (SF). The highest enzyme activity was achieved with P. chrysosporium after incubation for 7 days in a SSF process. The delignification processes were evaluated through enzymatic saccharification of pretreated substrate. The enzymatic saccharification of pretreated sugar cane bagasse was made using a commercial celullase previously characterized over an optimum substrate. It was determined that a pretreatment with P. chrysosporium in a SSF process for 14 days allowed a 20.81% increase in the fermentable sugars production. It was evaluated the optimal conditions for sugar cane bagasse enzymatic saccharification. It was established that the bagasse concentration has to be 5% w/v and it has to be incubated at 36 °C for 36 h. The bioethanol production from pretreated sugar cane bagasse was made in a simultaneous saccharification and fermentation process using S. cerevisiae like bioethanol producing microorganism. Thus obtained 3.2 g/L of ethanol with an YPS of 0.06 (g ethanol/g bagasse) and a fermentation efficiency of 59%.
Keywords:
bioethanol, enzymes, lignocellulose, pretreatment
Topic:
R&D on Processes for Solid, Liquid and Gaseous Fuels from Biomass
Subtopic:
Bioethanol production and sugar release from lignocellulosic biomass
Event:
21st European Biomass Conference and Exhibition
Session:
3DV.1.11
Pages:
1533 - 1538
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-3DV.1.11
Price:
FREE
