Title:
Characterization of Oligomers from Olive Tree Pruning by Hpaec, ESI/MS and Enzymatic Treatment
Author(s):
Álvarez, C., González, A., Ballesteros, I., Ballesteros, M., Negro, M.J., Oliva, J.M., Sáez, F.
Document(s):
Paper
Poster
Abstract:
Oligosaccharides constitute an important group of compounds with interesting applications in medicine and the food industry, as they have many healthy properties such as its prebiotic effect which encourages the growth of beneficial intestinal bacteria (Bifidobacterium and Lactobacillus). The liquid fraction obtained during steam explosion pretreatment of olive tree pruning contains hemicellulose with compounds from high molecular weight to monomeric soluble sugars. The aim of this work was the characterization of the oligomers obtained from soluble hemicellulose after the enzymatic treatment. A liquid fractionation was necessary to remove undesired compounds, which may interfere in the characterization of the oligosaccharides. To this end, various techniques have been used. First, gel filtration chromatography fractionated the sample, which enabled the removal of the monosaccharides, lignin and high molecular weight compounds. Secondly, size exclusion chromatography was used with columns of different cutting sizes (10000, 5000, 3000 Da), in order to estimate the range of polymerization degree present in the liquid fraction. High performance anion exchange chromatography with pulsed amperometric detection was subsequently used, which is a much more sensitive technique, showing a better distribution of the oligomers. In addition, in order to achieve the oligosaccharides with the desired degree of polymerization enzymes were used which were capable of breaking those compounds in a specified manner and release selectively oligosaccharides that can be used in the structural elucidation. The enzymatic hydrolysis was performed with aglucoamylase, endo(1,4)ßxylanase and Viscozyme L, achieving depolymerizations of the fractions. Mass spectrometry has been used to determine the molecular weight of the oligomers formed and to study the possible structures of these oligosaccharides. Oligomers of DP7pentoses and hexoses were detected through electrospray mass spectrometry.
Keywords:
enzymatic hydrolysis, pretreatment, olive tree pruning, oligosaccharides
Topic:
R&D on Biomass Conversion Technologies for Heating, Electricity and Chemicals
Subtopic:
Biorefineries
Event:
21st European Biomass Conference and Exhibition
Session:
2DV.2.42
Pages:
1060 - 1064
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-2DV.2.42
Price:
FREE
