Title:
Response of the Enzymatic Hydrolysis of Delignified Wood Using Commercial Cellulase
Author(s):
Ares, F., Pérez, G., Lopretti, M.
Document(s):
Paper
Poster
Abstract:
Nowadays, there is a great interest in using lignocellulosic materials as substrate for the production of ethanol. The present work evaluated the effect of enzyme loadings and substrate concentration on enzymatic hydrolysis of a mixture of wood chips, sawdust and shavings. Sawmill waste pretreated biologically was provided from Nalavip S.A. It consisted in a mixture (40:20:40) of wood chips, sawdust and shavings, respectively. For study different enzyme and substrate loadings, experiments were carried with the powder enzyme dissolved in 0.1 M acetate buffer (pH 4.8) and 1.1% (w/v) of substrate and 10 g/l of enzyme, respectively. The concentrations of enzyme studied were: 10, 20, 40, 80 and 120 g/L. The concentrations of substrate studied were: 1.1, 4.2, 5.7 and 7.1 % (w/v). The highest concentration of reducing sugars is given at 24 hours As we increase the enzyme concentration, production of reducing sugars and percentage of saccharification increases (16 kFPU/g of dry substrate, % saccharification=15,7; 195 kFPU/g dry substrate, % saccharification=88). We can conclude that the combination of biological and chemical methods is the most effective way for improve the production of reducing sugars. Enzyme loading and substrate concentration are variables of great influence on enzymatic hydrolysis of wood.
Keywords:
bioethanol, enzymatic hydrolysis, wood chips
Topic:
R&D on Processes for Solid, Liquid and Gaseous Fuels from Biomass
Subtopic:
Bioethanol production and sugar release from lignocellulosic biomass
Event:
21st European Biomass Conference and Exhibition
Session:
3DV.1.5
Pages:
1520 - 1522
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-3DV.1.5
Price:
FREE
