Title:
Cellulase Production in Packed Bed Bioreactor by Solid-State Fermentation
Author(s):
Casciatori, F.P., Casciatori, P.A., Thoméo, J.C.
Document(s):
Paper
Poster
Abstract:
Solid-state fermentation (SSF) is a process taking place in a solid matrix containing enough water to ensure microbial growth. This work addresses the process of cellulase production by the newly isolated thermophilic fungi Myceliophthora sp. I-1D3b by SSF in a packed bed bioreactor pilot-scale (3 in diameter and 1 m length). The main target is applying the enzymes for sugar release from lignocellulosic biomass in the enzymatic route of bioethanol production. Sugar cane bagasse (SCB) and wheat bran (WB) were used as substrates and the bioreactor was forcefully aerated. The highest temperature was near the bed top around 20 hours, but fortunately the increase of temperature was not significant. Substrate moisture content was reduced due to drying, but fungal development has occurred normally. Cellulase activities were very high, reaching 350, 500 and 4 units of activity per grams of dry solid substrate for endoglucanase, xylanase and filter paper activity, respectively. Hence, it is possible to say this process of cellulase production have been successfully scaled-up, because bioreactor thermal condition was stable and cellulolytic activities were satisfactory, although the substrate dried along the fermentation.
Keywords:
bioethanol, enzymes, fermentation, fixed bed, waste disposal, sugar cane bagasse
Topic:
R&D on Processes for Solid, Liquid and Gaseous Fuels from Biomass
Subtopic:
Bioethanol production and sugar release from lignocellulosic biomass
Event:
21st European Biomass Conference and Exhibition
Session:
3DV.1.13
Pages:
1539 - 1546
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-3DV.1.13
Price:
FREE
