Title:
Application of Flow Cytometry for Characterization of Biomass During ABE Fermentation Process
Author(s):
Tihomirova, K., Mezule, L., Juhna, T.
Document(s):
Paper
Abstract:
Production of acetone, butanol and ethanol (ABE) from the fermentation of clostridia is a common technology. However, reliable and rapid methods for selection of Clostridium spp. with the highest potential to convert enzymatically hydrolyzed cellulose materials to ABE are still not available. The aim of this study was to investigate the potential application of flow cytometry (FCM) to detect, enumerate and characterize Clostridium acetobutylicum, Clostridium beijerinckii and Clostridium tetanomorphum during ABE fermentation. During the study FCM staining protocol was optimized and pH effect evaluated due high pH range variation during ABE fermentation process (from about 7 to 4). The results from the fermentation showed that the data obtained with FCM are consistent with DAPI staining for total cell counts and cultivability data correlate with SYBR green and double stained cell counts (R2 = 0,838). The study showed that flow cytometry is rapid and sensitive enough for the monitoring of ABE fermentation on a routine basis.
Keywords:
biofuels, biomass, fermentation
Topic:
Biomass Resources
Subtopic:
Biomass feedstock, residues and by-products
Event:
20th European Biomass Conference and Exhibition
Session:
1BV.1.42
Pages:
344 - 349
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-1BV.1.42
Price:
FREE
