Title:
Fractionation of Oligomers from Pretreated Olive Tree Pruning and its Enzymatic Hydrolysis
Author(s):
Sáez, F., Álvarez, C., Ballesteros, I., Ballesteros, M., Manzanares, P., Negro, M. J., González, A., Oliva, J.M.
Document(s):
Paper
Abstract:
Olive tree pruning is a suitable raw material for ethanol production, and also an interesting biomass to obtain high value-added products such as oligosaccharides and antioxidants. It can have a positive effect on both the integral use of this agricultural residue and the improvement of ethanol yields. The aim of this study is to characterize and enzymatically hydrolyze the liquid fraction released during the steam explosion pretreatment of olive tree pruning. Steam-exploded olive tree pruning biomass was pretreated under 190 ºC for 25 minutes in a 2 L reactor. The prehydrolysate obtained from the hydrothermal treatment was purified in a HiLoad 16/60 Superdex 30 prep grade column. The three fractions collected were analyzed by high liquid size exclusion chromatography. Fraction F1 shows a molecular weight distribution of oligomers up to 20000 Da, F2 from 400 to 6000 Da and F3 contained low molecular weight compounds, monosaccharides and compounds with molecular weight up to 600 Da. The enzymatic hydrolysis of each of the fractions has been carried out with specific enzymes to depolymerize larger oligomers (glucoamylase, xylanase, cellulase) and thus to produce its selective hydrolysis. The highest proportion of oligosaccharides is in the first fraction with 43.4 % followed by fraction 2 with 33.6 %. The rest consists of monosaccharides and sugars with low degree of polymerization. The addition of enzymes to fractions F1 and F2 produces the oligomers hydrolysis to glucose, xylose, xylobiose, xylotriose and DP4.
Keywords:
enzymatic hydrolysis, hemicellulose, steam explosion, olive tree, HPSEC
Topic:
R&D on Biomass Conversion Technologies for Heating, Electricity and Chemicals
Subtopic:
Biorefineries
Event:
20th European Biomass Conference and Exhibition
Session:
2CV.1.16
Pages:
1202 - 1206
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-2CV.1.16
Price:
FREE
