Title:
Combination of Alkaline and Enzymatic Pre-Treatment to Increase Bio-Methane Production Potential of Sorghum and Wheat Straw
Author(s):
Rollini, M., Sambusiti, C., Musatti, A., Manzoni, M., Ficara, E., Malpei, F.
Document(s):
Paper
Abstract:
the purpose of this study was to increase methane production by enzymatic and combined alkaline¬enzymatic pre-treatments on ensiled sorghum forage and wheat straw. Sodium hydroxide pre-treatment was conducted by soaking samples in a NaOH solution. Enzymatic pre-treatment was comparatively performed employing four commercial preparations. Enzyme preparations were first characterized for their protein, CMCase (endoglucanase), xylanase and avicelase activities, and then added to untreated or alkaline pre-treated substrates. To assess the effect of the applied pre-treatment on the methane production, Biochemical Methane Potential (BMP) tests, before and after the enzymatic and the combined alkaline-enzymatic pre-treatment, were performed. Glucose was the prevailing monosaccharide released by enzymatic treatments, but xylose, mannose and galactose, as well as arabinose and glucuronic acid were also found. Combined alkaline-enzymatic pre-treatment resulted in the highest methane yield increase (+32% and +76% for ensiled sorghum and wheat straw respectively).
Keywords:
alkali, biogas, pretreatment, lignocellulosic sources, enzyme
Topic:
R&D on Biomass Conversion Technologies for Heating, Electricity and Chemicals
Subtopic:
Anaerobic digestion for biogas production
Event:
20th European Biomass Conference and Exhibition
Session:
2DV.3.5
Pages:
1406 - 1410
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-2DV.3.5
Price:
FREE
