Title:
Fast Growing Poplars in the Development of Plant Micropropagation System Based on Plant-Produced Ethylene Action
Author(s):
Žiauka, J., Kuusienč, S., Šilininkas, M.
Document(s):
Paper
Abstract:
Representatives of poplar genus, such as European aspen (Populus tremula L.) and its fast¬growing hybrids, are recognized as being among the most suitable tree species for short rotation coppicing in Northern Europe. In vitro culture is one of the tools for fast propagation of selected aspen genotypes. The present study was designed to test if the conditions suitable for increased accumulation of plant¬produced gas inside a culture vessel could contribute to commercially desirable changes in aspen development. Shoot cultures of several European and hybrid (P. tremuloides Michx. × P. tremula) aspen genotypes were studied using two different types of culture vessels. Even on the hormone¬free nutrient medium, aspen explants cultured in tightly sealed small¬volume Petri dishes (15 × 54 mm) formed between 1.7 and 6.7 times more shoots than in more voluminous test tubes (150 × 18 mm). The positive role of the gaseous hormone ethylene in shoot multiplication was confirmed by experiments with the ethylene¬releasing compound ethephon and ethylene signal inhibitor silver nitrate. Moreover, the shoots developed in small¬volume Petri dishes demonstrated superior rooting ability in the subsequent culture, suggesting that a plant micropropagation system based on the action of plant¬produced ethylene rather than of exogenous hormones is possible.
Keywords:
poplar, short rotation forestry (SRF), natural gas, plant hormone, shoot proliferation
Topic:
Biomass Resources
Subtopic:
Short rotation forestry and short rotation coppice
Event:
20th European Biomass Conference and Exhibition
Session:
1CV.3.7
Pages:
398 - 401
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-1CV.3.7
Price:
FREE
