Title:
Evaluation of Reusable Cellulase Activity in the Residue of Enzymatic Hydrolysis
Author(s):
Hasegawa, F., Inoue, H., Yokoyama, S., Imou, K., Yano, S.
Document(s):
Paper
Abstract:
One of the most effective ways to reduce the cost of hydrolysis is to reuse enzymes. To do this, it is important to know how much enzyme activity remains in the liquid phase or in the solid residue. In the solid residue, however, enzymes are absorbed onto the substrate and are difficult to completely separate from it, so it is not easy to estimate the activity by the same method as for the liquid phase. In this study, we proposed a method for evaluating the reusable cellulase activity in the residue of enzymatic hydrolysis and measured the cellulase activity by the method. 70% of cellulase activity remained in the solid residue after 70% of glucan hydrolyzed and 50% remained after nearly 90% hydrolyzed, thus cellulase was efficiently recovered from the hydrolysis residue. The cellulase activity in the solid residue after enzymatic hydrolysis measured by our proposed method was similar to that of the other experiments we conducted in this study and that reported by D. Lee et al. Therefore, our proposed method is efficient for evaluating cellulase activity in solid residue after enzymatic hydrolysis.
Keywords:
bioethanol, enzymatic hydrolysis, recycling
Topic:
R&D on Processes for Solid, Liquid and Gaseous Fuels from Biomass
Subtopic:
Bioethanol production and sugar release from lignocellulosic
Event:
20th European Biomass Conference and Exhibition
Session:
3DV.1.63
Pages:
1697 - 1699
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-3DV.1.63
Price:
FREE
