Title:
Oxidation of Glucose to Gluconic Acid and Regeneration of NADP Catalyzed by Glucose Oxidase and Dehydrogenases
Author(s):
Taraboulsi-Jr, F.A., Andreotti, D.Z., Yoriyaz, E.J., Vitolo, M.
Document(s):
Paper
Abstract:
This work dealt with the determination of catalytic activity and kinetic constants of glucose oxidase ( KM = 15.6 mM; Vmax = 34.1x10-3 mg H2O2/min.mL), glucose 6-phosphate dehydrogenase-NADP dependent (G6PDH; KM = 15.3 µM; Vmax = 0.110 µM/s) and glutamate dehydrogenase-NADPH dependent (GLUDH; KM = 0.052 µM; Vmax = 0.192 µM/s). It was shown that, through the discontinuous process (pH 5.0, 37oC and total duration of 90 min), the interchanging of the coenzymes (NADP and NADPH) occurred. The oxidation of glucose (32 g/L) by glucose oxidase (7.000 U) through a fed-batch process (carried out for 4h at pH 5.0 and 37oC) occurred at a yield of 80%. The volume inside the reactor was varied from 100 mL up to 400 mL by adding the glucose solution according to the equation: Vad = 0.15.t – 0.01875.t2 [Vad = added volume up to a given time (t)].
Keywords:
biotechnology, catalytic conversion, enzymatic process, fed-batch reactor
Topic:
Industrial Demonstration and Business Concepts
Subtopic:
Industrial biorefineries and bio-products
Event:
20th European Biomass Conference and Exhibition
Session:
4BV.4.20
Pages:
1972 - 1975
ISBN:
978-88-89407-54-7
Paper DOI:
10.5071/20thEUBCE2012-4BV.4.20
Price:
FREE
