Paper details

Title:

The Use of a Membrane Reactor for the NAD(H) Continuous Regeneration Catalyzed by NAD(H)-dependent Alcohol Dehydrogenase through the Substrate-coupled Procedure

Author(s):

Tomotani, E.J., Vitolo, M.

Document(s):

Abstract:

The potential of enzyme­catalyzed transformations is promising for the pharmaceutical and fine­chemical industries. Nonetheless, more complicated enzymatic reactions often require the presence of expensive cofactors (for instance, NAD, NADP). However it requires efficient regeneration of the nicotinamide cofactors to allow for an economically feasible process. The present work focuses on the applicability of alcohol dehydrogenase (ADH) from the yeast Saccharomyces cerevisiae, for regenerating the cofactor continuously using a Bioengineering Enzyme Membrane Reactor®. The process of cofactor regeneration was based on applying the reduction and the oxidation activities presented by ADH. The concentration of the substrate or the product was measured from the outlet samples collected at each residence time and the specific feeding rates used were 5, 30 and 60 mL.h­1. The optimum conditions for the reductive reaction were: 10 mM phosphate buffer (pH8.8) and its temperature being between 25­30°C; whereas for the oxidative reaction: 50 mM phosphate buffer (pH7.0) and its temperature being 35°C.

Keywords:

membrane reactor, alcohol dehydrogenase, continuous cofactor regeneration

Topic:

Biomass Conversion Technologies for Heating, Electricity and Chemicals                  

Subtopic:

Treatment for ethanol production

Event:

19th European Biomass Conference and Exhibition

Session:

OA9.4

Pages:

1015 - 1018

ISBN-13:

978-88-89407-55-4

ISBN-10:

88-89407-55-7

Paper DOI:

10.5071/19thEUBCE2011-OA9.4

Price:

FREE