Paper details

Title:

Kinetic Modeling of Pure Cellulases Involved in Enzymatic Hydrolysis of Cellulose

Author(s):

Chauve, M., Lopes Ferreira, N., Casanave, D., da Silva Perez, D., Perez, S.

Document(s):

Abstract:

The enzymatic hydrolysis of cellulose is still considered as a main limiting step of the biological production of biofuels from lignocellulosic biomass. This step involves the action of three types of cellulose degrading enzymes acting in a synergic way: Endoglucanases, Cellobiohydrolases and ß­glucosidases. This enzymatic cocktail, usually secreted by Trichoderma reesei at industrial scale, was already well studied and modelling the hydrolysis kinetic has been investigated. However, most of the models are empirical and few of them distinguish the kinetic activity of each enzyme. This article provides a new way to design a functional kinetic model dissociating the activity of ß­glucosidase as a final homogeneous reaction and the hydrolysis of cellulose by cellobiohydrolases in a heterogeneous phase. The predictive model combines a Michaelis­Menten (M­M) model for the ß­glucosidase action and a model based on Langmuir assumptions to describe the processive activity on solid substrate performed by cellobiohydrolases. Influences of temperature, enzyme and substrate concentration as well as influence of high contents of glucose have been studied and the suggested kinetic model allows a good prediction of all the experimental data.

Keywords:

bioethanol, cellulose, enzymatic hydrolysis, enzyme, modelling, modeling

Topic:

Biological conversion - Fermentation, enzymatic processes

Subtopic:

Biochemistry and microbiological communities

Event:

18th European Biomass Conference and Exhibition

Session:

OA6.5

Pages:

1380 - 1387

ISBN-13:

978-88-89407-56-1

ISBN-10:

88-89407-56-5

Paper DOI:

10.5071/18thEUBCE2010-OA6.5

Price:

FREE