Title:
Fermentation of Enzymatic Hydrolysate from Hydro-organo Pretreated Eucalyptus Globulus to Carotenoids and Triglycerides by the Red Yeast Rhodosporidium Toruloides
Author(s):
Caporusso, A., Morrone, N., Liuzzi, F., Albergo, R., Viola, E., Agrimi, G., Pisano, I., Di Fidio, N.
Document(s):
Paper
Poster
Abstract:
The transition towards a circular bioeconomy demands the integration of efficient lignocellulosic biomass pretreatment with a high-performing cell factory. In this study, Eucalyptus globulus residues were valorised through a sequential process combining an innovative hydro-organo pretreatment and enzymatic hydrolysis to efficiently release second-generation fermentable sugars. The resulting hydrolysate, rich in glucose and xylose, was subsequently employed as a carbon source for the oleaginous red yeast Rhodosporidium toruloides DSM 10134. Process optimisation, with particular emphasis on the carbon-to-nitrogen (C/N) ratio, enabled the coproduction of microbial oil and high-value carotenoids. Under the optimised process conditions, enzymatic hydrolysis reached 99 mol% and 95 mol% for glucose and xylose, respectively. Notably, at a C/N ratio of 60 g g-1, R. toruloides exhibited promising performance, achieving oil titers of 7.4 g L-1 alongside carotenoid production of 194 mg L-1. These findings highlight the potential of integrating chemical and biological processes to drive the transition towards a circular bioeconomy.
Keywords:
biorefinery, oleaginous yeast, single cell oils, lignocellulosic residue
Topic:
Biomass, Bio-based Products and Bioenergy Integration
Subtopic:
Integrated biorefineries for co-production of biofuels, biochemicals and bio-based products
Event:
34th European Biomass Conference and Exhibition
Session:
3CV.4.35
Pages:
506 - 510
ISBN:
978-88-89407-27-1
Paper DOI:
10.5071/34thEUBCE2026-3CV.4.35
Price:
FREE
