Title:
Microbial Lipid Production from Brassica carinata Residues: A Sustainable Feedstock for Single Cell Oil production
Author(s):
Caporusso, A., Liuzzi, F., Albergo, R., Giuliano, A., Panara, F., Bianco, L., Valerio, V., Viola, E., De Bari, I.
Document(s):
Paper
Poster
Abstract:
Microbial oils, commonly referred to as Single Cell Oils (SCOs), represent a promising sustainable feedstock for the production of biofuels and high-value chemicals. SCOs are synthesized by oleaginous microorganisms, including bacteria, fungi, microalgae, and yeasts. Among these, yeasts are particularly noteworthy for industrial applications. Depending on the substrate, these yeasts can accumulate lipids exceeding 20% of their dry cell weight (DCW). Additionally, they exhibit high growth rates on diverse carbon sources, require minimal land and water compared to oilseed crops, and offer ease of cultivation. This study focuses on the valorization of Brassica residues through a biorefinery approach, converting lignocellulosic sugars into SCOs using two oleaginous yeast strains: Lipomyces starkeyi and Trichosporon oleaginosus. The lignocellulosic biomass was characterized and subjected to steam explosion pretreatment to fractionate the three main structural components: cellulose, hemicellulose, and lignin. Subsequently, enzymatic hydrolysis of the structural polysaccharides was performed using the commercial blend Cellic CTec3. The resulting hydrolysate was used as a medium for lipid production by two yeast species, Lipomyces starkeyi and Trichosporon oleaginosus. Under optimized conditions the intracellular lipid content reach 49 and 39% of dry cell weight, respectively for L. starkeyi and T. oleaginosus.
Keywords:
biorefinery, oleaginous yeast, single cell oils, lignocellulosic residue
Topic:
Biomass Conversion to Intermediate Bioenergy Carriers and Sustainable Biofuels
Subtopic:
Biofuels and renewable hydrocarbon biofuels
Event:
34th European Biomass Conference and Exhibition
Session:
5AV.4.24
Pages:
857 - 862
ISBN:
978-88-89407-27-1
Paper DOI:
10.5071/34thEUBCE2026-5AV.4.24
Price:
FREE
