Title:
Identification of Biocatalysts in an Enriched Population of Hydrogen Fermenting Bacteria
Author(s):
Piccione, B., Liuzzi, F., Bianco, L., Alagna, F., Lopez, L., Fantini, E., Daddiego, L., Facella, P., Villone, A., Fasano, C., Cangiulli, G., Montemurro, C., Panara, F.
Document(s):
Paper
Poster
Abstract:
A protocol for the enrichment of homoacetogenic microorganisms was developed to select strains capable of hydrogen fixation and CO2 reduction. Using digestate from an industrial biogas plant as inoculum, cultures were incubated under autotrophic conditions (H2:CO2 = 80:20, pH ~6.8, 37°C) and monitored for pressure, gas composition, and production of acetate and ethanol. Over successive subcultures, microbial communities enriched in hydrogen-fermenting bacteria were selected. The highest acetate yield recorded was 78.6 mmol/L after 738h. Metagenomic analysis revealed dominance of Clostridium and Sporomusa spp., known to synthetize key enzymes such as formate dehydrogenases and putative hydrogen-dependent CO2 reductases. This study demonstrates the feasibility of isolating and characterizing microbial consortia for bio-based hydrogen valorisation.
Keywords:
anaerobic digestion, enzyme, bacteria
Topic:
Biomass Conversion to Intermediate Bioenergy Carriers and Sustainable Biofuels
Subtopic:
Biofuels and synthetic fuels from biomass and hydrogen
Event:
33rd European Biomass Conference and Exhibition
Session:
5BV.6.20
Pages:
990 - 992
ISBN:
978-88-89407-25-7
Paper DOI:
10.5071/33rdEUBCE2025-5BV.6.20
Price:
FREE
