Title:
2,3-Butanediol Production from Saccharified Agri Food Mixed-biowaste by Recombinant Escherichia Coli Strain
Author(s):
Tomas-Tenes, G., Valles, A., Escrich, A., Vivo-Llorca, G., Torrejón-Cabello, A.
Document(s):
Paper
Slide presentation
Abstract:
The agricultural industry generates substantial amounts of mixed bio-waste, much of which is rich in organic matter and valuable components but often discarded. Efficient processing and valorization of these plant-based residues represent a valuable opportunity to create high-value compounds. In this study, three secondary streams were nutritionally characterized coming from different feedstocks: wholesale markets (MCV), food and drink industry (NdM) and greenhouses (SACh). Saccharification was carried out, resulting in conversion yields up to 59%, 68% and 43% (w/w) for MCV, NdM and SACh biowaste, equivalent to 20; 15 and 12g/L sugar release, respectively, to be used as the sole carbon source for the production of 2,3-butanediol (2,3-BDO) using a recombinant E. coli strain. Metabolic engineering was performed in different E. coli strains to express the Klebsiella oxytoca a-acetolactate synthase (BudB), a-acetolactate decarboxylase (BudA) and 2,3-butanediol dehydrogenase (BudC) genes. Different culture media compositions were designed, applying different sugar sources obtained by the previous saccharification. In shake flask experiments, the production yield of 2,3-BDO reached a maximum of 3,9 g/L, equivalent to 0,1g of 2,3-BDO per g of glucose, 43 times higher than the obtaining using a commercial culture media. Finally, fermentation in fedbach mode resulted in a final concentration of 50,3g/L.
Keywords:
fermentation, agroindustrial residues, hydrolysis, biobased products
Topic:
Biomass Conversion to Biomaterials and Chemicals
Subtopic:
Processes for biomaterials and bio-based chemicals
Event:
33rd European Biomass Conference and Exhibition
Session:
6BO.5.1
Pages:
1077 - 1080
ISBN:
978-88-89407-25-7
Paper DOI:
10.5071/33rdEUBCE2025-6BO.5.1
Price:
FREE
