Title:
Enhanced Co-production of Lipids and Biochemicals from Oleaginous Yeasts and Microalgae Co-cultivation Using Waste Glycerol Substrates
Author(s):
Khalid, U., Pittman, J., Theodoropoulos, C.
Document(s):
Paper
Abstract:
Microbial lipids production has emerged as a sustainable method for next genration biofuels by offering a renewable alternative to fossil fuels. In this work, co-cultivation of Yarrowia lipolytica (DSM 70562) and Chlorella vulgaris (CCAP 211/11B) has been carried out under mixotrophic conditions by using modified Bold’s Basal Medium (BBM) supplemented with peptone, glycerol and yeast extract. Parameters optimisation was performed to maximise the lipids production inlcuding inoculum ratio and glycerol concentration change. Inoculum ratios ranging from 1:1 to 1:16 were tested, with 1:4 yeast to algae ratio providing optimal growth. Increase in the glycerol concentration upto 20 g/L in BBM-GP media futher increased the production, reaching at 9.68.±.0.3.g/L dry biomass and 2.92.±.0.2.g/L lipids after 120 hours of fermentation. The co-culture has also produced some extracellular polyols such as mannitol, arabitol and erythritol, making mutualistic interactions stronger. Algal contribution was measured via chlorphyll a/b content, and GC-FID analysis has shown balanced contribution of monounstaurated fatty acids (C16:1 and C18:1) and polyunsaturated fatty acids (C18:2, C18:3), thus, underscoring the potential of this system for biodiesel production from low-cost substrate.
Keywords:
biofuel, glycerol, co-cultivation, polyalcohols
Topic:
Biomass Conversion to Biomaterials and Chemicals
Subtopic:
Co-production of biofuels and biochemicals
Event:
33rd European Biomass Conference and Exhibition
Session:
6BV.10.4
Pages:
1125 - 1129
ISBN:
978-88-89407-25-7
Paper DOI:
10.5071/33rdEUBCE2025-6BV.10.4
Price:
FREE
