Title:
Harnessing Acetate from Syngas as Substrate for Microbial Platforms in Polyhydroxyalkanoates Production
Author(s):
Ciliberti, C., Caporusso, A., Albergo, R., Agrimi, G., De Bari, I., Pisano, I.
Document(s):
Paper
Poster
Abstract:
This study reports the development of microbial cell factories able to produce polyhydroxyalkanoates (PHAs) by using renewable and low-cost feedstocks, such as acetate obtained through syngas fermentation. The process involves two steps in which first Clostridium aceticum (DSM 1496) was employed to convert syngas mixes into acetate and second acetate was used as substrate for Cupriavidus necator (DSM 545) for its conversion into PHAs. The optimization of the process using simulated syngas mixes with C. aceticum, achieved conversion yields of 38% (YP/S) under optimal conditions. Subsequently, a simulated medium supplemented with acetate was used for C. necator cultivations testing different cells inoculum sizes (0.7, 1.4 and 2.7 g L-1 dry cell weight L-1) and initial medium pH (pHi 8 and 5). Results indicated that increasing cell inoculum led to a linear increase in PHAs production, as well as more acidic medium. The highest PHAs production was observed with a cell inoculum of 2.6 g L-1 dry cell weight L-1 at an initial pH 5, resulting in a 2-fold increase compared to the initial pH 8. These preliminary results demonstrate the potential of microbial cell factories for acetate valorisation.
Keywords:
biorefinery, biobased products, biopolymers, syngas fermentation, polyhydroxyalkanoates
Topic:
Biomass Conversion to Bio-based Products and Chemicals
Subtopic:
Biorefinery platforms for bio-based chemicals and polymers
Event:
32nd European Biomass Conference and Exhibition
Session:
6CV.9.23
Pages:
870 - 874
ISBN:
978-88-89407-24-0
Paper DOI:
10.5071/32ndEUBCE2024-6CV.9.23
Price:
FREE
