Title:
Conversion of Lignocellulosic Biomass and Side-stream Products into Microbial Oils
Author(s):
Caporusso, A., De Bari, I., Albergo, R., Liuzzi, F., Valerio, V., Viola, E., Giuliano, A., Braccio, G.
Document(s):
Paper
Slide presentation
Abstract:
This work analyzed different feedstocks, namely lignocellulosic biomass and raw glycerol, as suitable carbon sources to produce single cell oils. Different lignocellulosic hydrolysates were produced by ENEA through steam pre-treatment at optimized conditions followed by enzymatic hydrolysis at high dry matter consistency. Cardoon residues, giant reed and wheat straw have been explored over time as different raw materials to produce hydrolysates. Microbial conversion to SCO were carried out by Lipomyces tetrasporus Li-0407, Cryptococcus curvatus CA-3802, and Yarrowia lipolytica ATCC 46483. Besides the typical process parameters affecting the microbial growth and fatty acids profile, such as the medium composition, the carbon sources concentration, and the nitrogen sources and C/N ratio, also the inoculum age and size can significantly affect the process yields. Fermentation trials were conducted in stirred flask and lab-scale bioreactors. Optimized process conditions were scaled up in a 50 L bioreactor pilot plant. This allowed for more effective control of the process conditions and the extraction of appropriate amounts of oils to be tested in target applications. Techno-economic evaluations were finally implemented.
Keywords:
biodiesel, biorefinery, glycerol, techno-economic analysis, microbial oils, lignocellulosic sugars
Topic:
Biomass Conversion to Bio-Based Products and Chemicals
Subtopic:
Processes for bio-based chemicals and materials
Event:
31st European Biomass Conference and Exhibition
Session:
6BO.8.3
Pages:
1033 - 1037
ISBN:
978-88-89407-23-3
Paper DOI:
10.5071/31stEUBCE2023-6BO.8.3
Price:
FREE
