Title:
Birch Bark Based Suberinic acid Sample Fractionation and Characterisation Using Instrumental Methods
Author(s):
Godina, D., Feldmanis, A.R., Makars, R., Paze, A., Rizikovs, J.
Document(s):
Paper
Abstract:
Suberin is a lipophilic (hydrophobic) macromolecule that is found in the cell walls of the outer layer (bark) of many plants and protects the plant from environmental conditions and pests. It is a complex polyester composed of linear, polyfunctional long carbon chain (predominantly C18) suberin fatty acids (SA) and glycerol, which, in turn, in the birch outer bark, is covalently linked to lignin monomer-like polyphenols, which give it a dark colour. When depolymerisation of suberin in an alkaline medium is performed, a mixture of various SA is obtained. There is a lack of published research based on SA isolation and complete characterisation, which is important for further usage. In these experiments, isolated and fractionated birch outer bark from AS Latvijas Finieris plywood mill (Latvia) was employed. The birch outer bark was extracted with ethanol before depolymerisation to eliminate extractives (triterpenoids, phenolic compounds, etc.). This research was based on the SA sample full characterisation by performing SA solubility determination in solvents with increasing polarity, SA sample fractionation using different methods - fractionation with size exclusion chromatography system and ion exchange chromatography and comprehensive characterization of obtained SA fractions using various instrumental analytical methods.
Keywords:
biomass, biorefinery, fractionation, birch bark, suberin, solubility, suberinic acids
Topic:
Biomass Conversion to Bio-Based Products and Chemicals
Subtopic:
Processes for bio-based chemicals and materials
Event:
31st European Biomass Conference and Exhibition
Session:
6AV.6.6
Pages:
1043 - 1049
ISBN:
978-88-89407-23-3
Paper DOI:
10.5071/31stEUBCE2023-6AV.6.6
Price:
FREE
