Title:
Immobilized Enzymatic Process for the Production of Fructose from Inulin-Rich Cardoon Roots
Author(s):
Liuzzi, F., Viola, E., Valerio, V., De Bari, I.
Document(s):
Paper
Poster
Abstract:
Inulin is a reserve carbohydrate accumulated in the roots and tubers of many plants belonging mainly to the Asteraceae family, including Cardoon (Cynara cardunculus L.). The hydrolysis of inulin allows to obtain fructose, a starting monomer to obtain key molecules for biobased platforms for the production of biomaterials and renewable biofuels. The use of acidic agents in the hydrolysis process is unfavorable from an environmental point of view and is corrosive for industrial plants, therefore the research turns its attention to enzymatic hydrolysis. The enzymes used for this purpose are called inulinases and have the disadvantage of being expensive, so a strategy that can be used to make the process more cost-effective is to reuse the enzyme by immobilizing it. The aim of the present work was to maximize the fructose yield from cardoon by using immobilized inulinase on epoxy media. The results showed a conversion yield of 87% after the treatment of inulin batches, allowing to save about three-fold enzyme compared to free-inulinase methods.
Keywords:
biorefinery, enzymatic hydrolysis, immobilization, inulinase, cardoon roots
Topic:
Biomass, Bio-Based Products and Bioenergy Integration
Subtopic:
Biomass use in biorefineries
Event:
30th European Biomass Conference and Exhibition
Session:
3CV.6.21
Pages:
505 - 508
ISBN:
978-88-89407-22-6
Paper DOI:
10.5071/30thEUBCE2022-3CV.6.21
Price:
FREE
