Title:
Bioprofiling and Isolation of Succinic Acid Producing Bacteria from Cow Dung for their Adaptation in 'One-pot' Bioconversion of Corn Cob to Succinic Acid
Author(s):
Moshokoa, M.F., Daramola, M.O., Adeleke, R.A., Ndaba, B., Roopnarain, A.
Document(s):
Paper
Abstract:
Succinic acid is a platform chemical that is used as a precursor for various applications It is currently produced via. the fermentation process, which is sustainable. To make the process of fermentation cost effective, this research is aimed at bioprospecting and isolating succinic acid producing bacteria within cow dung for one-pot bioconversion of corn cob to succinic acid. Organic acid producing bacteria were screened and successfuly isolated. Isolates which produced organic acids were 29 and only 12 were chosen for further studies. The organic acids were identified and quantified using High Performance Liquid Chromatographt. The organic acids quantified were malic, acetic, succinic, lactic and formic acids. Next generation sequencing results showed presence of putative succinic acid producing and succinic acid consuming bacteria. The predominant phyla found was proteobacteria at 36,02%, followed by Firmicutes at 27,43% and Bacteroidetes at 22,08%. Succinic acid producing bacteria were successfully isolated within cow dung. Formic acid was the highest organic acid produced across all 12 selected isolates, followed by succinic acid.
Keywords:
bacteria, corn cob, cow dung, next generation sequencing, succinic acid
Topic:
Biomass Conversion to Bio-Based Products and Chemicals
Subtopic:
Processes for bio-based chemicals and materials
Event:
30th European Biomass Conference and Exhibition
Session:
6BV.2.12
Pages:
1107 - 1110
ISBN:
978-88-89407-22-6
Paper DOI:
10.5071/30thEUBCE2022-6BV.2.12
Price:
FREE
