Title:
Bioaugmentation Strategies to Enhance Methane Production from Lignocellulosic Substrates: Dynamics of the Prokaryotic Community Structure
Author(s):
Obi, L.U., Tekere, M., Roopnarain, A., Adeleke, R.A.
Document(s):
Paper
Abstract:
Optimization of anaerobic digestion (AD) of lignocellulosic substrates via bioaugmentation for enhanced biomethane production is imperative. Here, eight different screened bacterial isolates were used to augment different batch anaerobic digesters that comprised freshly chopped water hyacinth (WH) and cow dung (CD), at 2% total solids (w/v). Methane production was monitored at intervals and DNA metabarcoding of the 16S rRNA genes revealed the dynamics of the prokaryotic community structure of the anaerobic digesters. Obtained results suggest bioaugmentation improved the abundance and diversity of the prokaryotic community. The treatment that was inoculated with Serratia marcescens produced the highest cumulative methane of 0.68 L, 45.6% methane more than the consortium treatment. A shift from Pseudomonas to Bacteroides was observed in the bacterial community at the genus taxonomic level after AD while Methanosarcina dominated the archaeal genera. Furthermore, independent bioaugmentation of AD of different substrates such as water hyacinth, cow dung, and cellulose powder with identified model bioaugmentation agent, Serratia marcescens portrayed a positive effect with regards to methane production when compared with the control treatment. This study represents an economic and environmentally friendly approach of single isolate inoculation (bioaugmentation) in optimizing methane production from lignocellulosic substrates such as water hyacinth.
Keywords:
methane, bioaugmentation, water hyacinth, lignocellulosic, Serratia marcescens, prokaryotic
Topic:
Biomass Conversion for Bioenergy
Subtopic:
Anaerobic digestion for biogas and biomethane production
Event:
30th European Biomass Conference and Exhibition
Session:
4CV.10.3
Pages:
807 - 818
ISBN:
978-88-89407-22-6
Paper DOI:
10.5071/30thEUBCE2022-4CV.10.3
Price:
FREE
