Title:
Dilute Acid Hydrolyzed Soybean Meal for Cultivation of Isolated Clostridium sp. G10
Author(s):
Sanguanchaipaiwong, V., Sabua, C., Hemnusoornnanon, N., Nuangpanom, P., Leksawasdi, N.
Document(s):
Paper
Poster
Abstract:
Soybean meal is a by-product from soybean oil manufacturing. It contained, on the mass basis, crude fiber (6.3%) and protein (47.6%) after oil extraction. The aim of study was to investigate the cultivation of isolated Clostridium sp. G10 using soybean meal hydrolysate as a N- and C-source in fermentation medium. Soybean meal hydrolyzed with 0.02 to 0.1 M of HCl and H2SO4 and was heated at 121 °C, 15 psi for 10-30 min. The result showed that hydrolyzing soybean with 0.1 M HCl and 20-min heating period were the optimal conditions to obtain soluble protein (24.45 ±2.21 g/L) and reducing sugar (13.12 ±1.09 g/L). To study the effect of N- and C-sources replacement in T6 medium, soybean meal hydrolysate was utilized to replace yeast extract and tryptone resulting in 8 g/L soluble protein and used as a C-source with the addition of glucose with 50 g/L reducing sugar equivalent. The butanol concentration (10.80 ±0.44 g/L) was slightly lowered than that of control T6 medium (12.15 ±0.07 g/L). These results demonstrated that Clostridium sp. was able to uptake the N-and C-sources contained in the hydrolyzed soybean meal. However, a C-source alternative is required to mitigate the cost of raw materials.
Keywords:
fermentation, agroindustrial residues, anaerobic process, biobutanol, Clostridium sp.
Topic:
Biomass Conversion to Intermediate Bioenergy Carriers and Sustainable Biofuels
Subtopic:
Bio-alcohols from sugars, starch and lignocellulosic biomass
Event:
30th European Biomass Conference and Exhibition
Session:
5BV.6.14
Pages:
927 - 931
ISBN:
978-88-89407-22-6
Paper DOI:
10.5071/30thEUBCE2022-5BV.6.14
Price:
FREE
