Title:
Somaclonal ckx-Downregulated Line of Finger Millet as a Feedstock for Sustainable Bioethanol Production
Author(s):
Blume, R.Y., Yemets, A.I., Rakhmetov, D.B., Blume, Y.B.
Document(s):
Paper
Slide presentation
Abstract:
Bioethanol is considered at the moment as one of the most promising substitutes for petroleum. Finger millet (Eleusine coracana) has recently gained significant attention as a bioethanol feedstock. Earlier, we were able to create several somaclonal lines of this crop, one of which (SE-7 or cv. Yaroslav-8) with decreased level of cytokinin-degrading enzyme expression (cytokinin oxidase/dehydorgenase, ckx), what significantly promotes biomass accumulation of this variety. In this study, we aimed to evaluate bioethanol production potential from straw and grains of finger millet varieties bred conventionally and from somaclonal variants. It was confirmed that somaclonal lines are showing same beneficial traits under field conditions (reduced height, increased tillering, etc.). Higher biomass/grain productivity in SE-7 line was observed (30% more than in its wild-type cv. Tropikanka). We have estimated that it is possible to produce up to 2021 L/ha of ethanol from seeds and up to 1802 L/ha from straw. Total ethanol output can reach 3823 L/ha (SE-7). Somaclonal line SE-7 with naturally downregulated ckx2 has outperformed by potential ethanol yield parental line by at least 33.4%.
Keywords:
bioethanol, biomass, straw, somaclonal variability, Eleusine coracana, finger millet
Topic:
Sustainable Resources for Decarbonising the Economy
Subtopic:
Biomass Crops and Energy Grasses
Event:
29th European Biomass Conference and Exhibition
Session:
1BO.7.3
Pages:
81 - 84
ISBN:
978-88-89407-21-9
Paper DOI:
10.5071/29thEUBCE2021-1BO.7.3
Price:
FREE
