Title:
'One-Pot' Bioconversion of Corn Cob to Succinic Acid Using a Molten Hydrate Salt Pretreatment Slurry by Actinobacillus Succinogenes
Author(s):
Ejekwu, O., Ayeni, A.O., Sadare, O., Mokwatlo, S.C., Daramola, M.O.
Document(s):
Paper
Slide presentation
Abstract:
Efficient bioconversion of lignocellulosic biomass to platform chemicals is important for the overall viability and adoption of sustainable bioprocesses. A novel “one pot” production of succinic acid by Acti- nobacillus succinogenes from waste corn cob incorporating pretreatment and bioconversion in one vessel was developed. This unit offers the advantage of a more cost effective and robust bioconversion process. This study aims to analyse the effect of pretreatment type, pretreatment volume, pH and MgCO3 supplementation on the production of succinic acid from corn cob by a single succinic acid producing organism, Actinobacillus succinogenes in the ”one-pot” bioconversion configuration. The fermentation medium was added at a volume of 50mL and pH of 7.2 to the pretreatment liquor and the process was run for 72h. The fermentation samples was analysed with HPLC to determine the concentration of succinic acid and by-product formed. The results showed that ZnCl2.4H2O pretreatment of corn cob (10g/LZnCl2.4H2O, 12% (v/v) solid:liquid ratio, 70°C; 60-minpretreatment) was superior compared to ZnCl2.4H2O/Urea method. The volume of the pretreatment affected the metabolite profile of the fermentation broth with the highest succinic acid concentration observed at 10 g and substrate inhibition as well as mass transfer limitations observed in higher loading volumes. The research further develops the use of waste corn cob biorefinery approach using a one-pot configuration.
Keywords:
biorefinery, fermentation, waste, pretreatment, enzymatic process, biobased economy
Topic:
Biomass Conversion to Intermediate Bioenergy Carriers, Sustainable Biofuels and Bio-Based Products
Subtopic:
Bio-Based Chemicals and Materials
Event:
29th European Biomass Conference and Exhibition
Session:
3AO.3.3
Pages:
691 - 698
ISBN:
978-88-89407-21-9
Paper DOI:
10.5071/29thEUBCE2021-3AO.3.3
Price:
FREE
