Title:
Rice Husk Biological Pre-treatment with Pleurotus Ostreatus
Author(s):
Cruz, L., González, C., Franco, K., Duran, D., Chirivi, J., Sierra, R.
Document(s):
Paper
Poster
Abstract:
Semi-solid culture has shown a convenient control of the cultivation as well as high laccase activities. Thus, in this work, P. otreatus was used in biological pre-treatment of rice husk using a semi-solid culture. . A selectivity to hemicellulose degradation by P. ostreatus was found. Nevertheless, it changed to cellulose after day 15. Exo-glucanase activity was the larger observed of hydrolases with an average maximum value of 1,300 U/L. Thus, in order to avoid cellulose degradation in pre-treatment with P. ostreatus it should be stopped at day 15. Additionally, fungal biomass profile showed a logistic behavior related at first with space limitations in the flask. Furthermore, in aqueous extracts reducing sugars concentration were up to 13 g/L. On the other hand, the aqueous extracts obtained in semi-solid culture showed a dephenolization capacity. Accordingly, this sub-product of pre-treatment is suitable in bioremediation applications. Finally, an inhibition resistance of P. ostreatus was related to higher laccase activity and dephenolization capacity. The reached delignification in 23 days of pretreatment was around 9%, and biomass digestibility of rice husk increased 57%. This work, suggests high laccase activity is not necessarily high delignification percentages in pre-treatment.
Keywords:
biodegradability, lignin, lignocellulose, rice husks, losses
Topic:
Biomass Conversion Technologies for Energy Carriers, Chemicals and Materials
Subtopic:
Bio-alcohols from lignocellulosic biomass and pretreatment
Event:
27th European Biomass Conference and Exhibition
Session:
3CV.7.17
Pages:
1364 - 1371
ISBN:
978-88-89407-19-6
Paper DOI:
10.5071/27thEUBCE2019-3CV.7.17
Price:
FREE
