Title:
Co-culture of White-rot Fungi in Rice Husk and Sugar Cane Bagasse for Laccase Production and Delignification
Author(s):
Cárdenas-Bustos, C.S., Villarreal, C., Prieto-Vivas, J.E., Delgado, S., Sierra, R., Chiriví-Salomón, J.S.
Document(s):
Paper
Abstract:
White-rot fungi are the world-renown producers of laccases, and lignocellulose present in their substrate culture can induce their expression. Strategies to increase laccases secretion and delignification are desired due to their industrial applicability in bioremediation, renewable energy production, and the textile industry, among others. Nevertheless, to obtain high laccase activity by fungi, long times are required. Therefore, co-cultures have been proposed as a strategy to increase laccase production because competition for the substrate may motivate them to reach their highest potential. In this study, in addition to co-cultures, two substrates were tested: rice husk and sugarcane bagasse. Pleurotus ostreatus monoculture using rice husk as substrate performed the greatest laccase activity reaching 21,000 U per L. This result suggests that a co-culture is not required for this substrate. However, when sugarcane bagasse was the substrate, a co-culture of P. ostreatus with Pleurotus eryngii showed the highest activity: 18,944 U/L. Productivity was calculated as indicator for the time required to reach highest peak in activity. The co-culture of Trametes pubescens and P. ostreatus exhibited the highest productivity in both substrates (1,184 and 630 U/L·d for rice husk and sugarcane bagasse, respectively). Contrastingly, all cultures with Lentinula edodes showed a decrease in laccase activity, indicating an antagonistic interaction in mixed cultures. In addition, the glucose deployment in the culture medium stimulated laccase production, showing the nutritional dependence to produce ligninolytic enzymes. On the other hand, higher delignification values were expected in cultures that produced laccases with greater activities, but no correlation between lignin degradation and laccase productivity was observed, which could be related with . This may be due toa substrate specificity for each fungus. This is the first experimental design which studies laccase productivity and delignification in more than four white-rot fungi. Biochemical interaction among enzymes and metabolites from co-culture secretomes should be deeply understand to enhance laccase productivity or delignification
Keywords:
competition, Ganoderma Lucidum, laccase productivity, Lentinula Edodes, Pleurotus Spp, Trametes Pubescens
Topic:
Biomass Conversion Technologies for Energy Carriers, Chemicals and Materials
Subtopic:
Bio-alcohols from lignocellulosic biomass and pretreatment
Event:
27th European Biomass Conference and Exhibition
Session:
3CV.7.13
Pages:
1351 - 1359
ISBN:
978-88-89407-19-6
Paper DOI:
10.5071/27thEUBCE2019-3CV.7.13
Price:
FREE
