Title:
Laccase Production in Rice Husk by Pleurotus Ostreatus and Trametes Pubescens in Co-culture for Dephenolization
Author(s):
Cárdenas-Bustos, C.S., Cajiao, A., Manrique-Gonzalez, P., Sierra, R., Chiriví-Salomón, J.S.
Document(s):
Paper
Abstract:
Laccases are multicopper oxidases that catalyze the oxidation of phenolic compounds. Thereby, they have potential to be applied for bioremediation of waste streams. Laccases from white-rot fungi have shown significant removal of phenol, but no assays from laccases produced in co-culture have been reported. Experimental design was performed to elucidate optimal pH and temperature conditions. In this research, dephenolization percentages >60 were achieved using laccases obtained from monocultures of Pleurotus ostreatus, Trametes pubescens, and their co-culture. In the pH and temperature conditions tested, dephenolization using extracts obtained from monocultures resulted to be highly variable along tested conditions. In contrast, dephenolization obtained from co-culture extracts registered values above 65 %. Co-culture extracts are both efficient and robust along different pH and temperature conditions, thereby very suitable for industrial applications. Studies over decreasing time of reaction complement the desire optimization over the biological dephenolization process proposed in this study.
Keywords:
thermostability, pH stability, phenol oxidation, crude extracts
Topic:
Biomass Conversion Technologies for Energy Carriers, Chemicals and Materials
Subtopic:
Production and application of biobased chemicals
Event:
27th European Biomass Conference and Exhibition
Session:
3DV.4.22
Pages:
1445 - 1449
ISBN:
978-88-89407-19-6
Paper DOI:
10.5071/27thEUBCE2019-3DV.4.22
Price:
FREE
