Title:
Biomass Production Potential of Chlorella Pyrenoidosa Using Olive-mill Wastewater, and Carbon Dioxide Biofixation
Author(s):
Maaitah, M., Hodaifa, G., Malves, A., Sánchez, S.
Document(s):
Paper
Poster
Abstract:
The growth response of Chlorella pyrenoidosa was studied using a concentration of olive-mill wastewater (waters from oils washing) as culture media formed by dilution of the OMWW (30% v/v), with different CO2 concentrations (1%, 2%, 5%, 10% and 15%), temperature 25°C, pH 8.0, air flow was of 1 v/v/ min, and under light dark cycle (12 h lighting per day). All experiments were conducted with bioreactors of 0.5 dm3.This study summarizes how CO2 levels affected microalgal C. pyrenoidosa, considering the evaluation of the growth kinetics (maximum specific growth rate, µm, and volumetric biomass productivity, Pb) as well as the formation of lipids in the biomass formed. At lab level, a concentration of 30% (v/v) of olive-mill wastewater, and a concentration of 10% CO2 in the gas phase, permits to reach the highest values of µm (0.034 h-1) and Pb (8.6 10-3 g dm-3 h-1).In relation to the biochemical composition, the higher values of productivities in total chlorophyll (1.73 %), carotenoids (0.39 %), and protein (42.96 %), as well as a high concentration of total lipids (48.65%), were obtained in cultures that used a concentration of 10% CO2, being this concentration of lipid suitable for biofuel production. Oil samples collected from C. pyrenoidosa were converted into fatty acid methyl ester (FAME) through a transesterification process. The fatty acid profiles obtained are suitable for biofuel production. It is necessary to highlight that the percentages of polyunsaturated and essential fatty acids increased with the percentage of CO2in the air, and this implies greater fixation of CO2 when C. pyrenoidosa was used.
Keywords:
biofuel, biomass, treatment, olive mill wastewaters, chlorella pyrenoidosa, lipidis, bio-fixation
Topic:
Biomass Resources
Subtopic:
Algae production systems
Event:
27th European Biomass Conference and Exhibition
Session:
1DV.1.1
Pages:
236 - 240
ISBN:
978-88-89407-19-6
Paper DOI:
10.5071/27thEUBCE2019-1DV.1.1
Price:
FREE
