Title:
Is it Possible to Produce Low-nitrogen Hydrolysate of Giant Reed Suitable for Single Cell Oil Production?
Author(s):
Cianchetta, S., Polidori, N., Ceotto, E., Galletti, S.
Document(s):
Paper
Poster
Abstract:
Single cell oil (SCO), obtainable from the fermentation of lignocellulose hydrolysates by oleaginous microorganisms, represents a promising and sustainable option for biorefinery (biodiesel, biolubricants or other biobased chemicals). SCO production requires low-nitrogen and sugar-rich substrates (high C/N medium). Giant reed (Arundo donax) is a perennial lignocellulosic grass, with low input requirements and a very high yield of biomass, that could be converted in SCO, after opportune pretreatment and hydrolysis. However, this biomass is relatively recalcitrant to enzymatic hydrolysis, and harsh pretreatments may release inhibitors for the microorganism growth. The aim of this work was to obtain a giant reed hydrolysate suitable for a high-yield SCO production by the yeast Lipomyces starkeyi. An enzymatic hydrolysate was obtained from winter-harvested biomass of giant reed, after mild alkaline pretreatment, with a C/N ratio of 196. The hydrolysate allowed to obtain dry cell biomass of L. starkeyi with a very high lipid content (up to 71±3 % w/w of total lipids). The composition of the SCO obtained (oleic acid 41%, saturated fraction above 50 %) is compatible with the production of biodiesel
Keywords:
alkali, biodiesel, enzymatic hydrolysis, pretreatment, Lipomyces Starkeyi, Arundo donax
Topic:
Biomass Conversion Technologies for Energy Carriers, Chemicals and Materials
Subtopic:
Production and application of biobased chemicals
Event:
27th European Biomass Conference and Exhibition
Session:
3DV.4.7
Pages:
1421 - 1423
ISBN:
978-88-89407-19-6
Paper DOI:
10.5071/27thEUBCE2019-3DV.4.7
Price:
FREE
