Title:
Value Enhancement of Microalgae Utilization Employing Mild Extraction and Hydrothermal Liquefaction for Protein and Bio-Crude Production
Author(s):
Kuhavichanan, A., Kusolkumbot, P., Kaharn, J., Suemanotham, A., Sirisattha, S., Areeprasert, C.
Document(s):
Paper
Poster
Abstract:
The two-stage conversion strategy of microalgae, consisting of protein extraction and conversion of extracted residue into bio-crude, was adapted to enhance value chain of microalgae utilization in this work. The sets of experiment have been done starting from the extraction of protein from fresh microalgae followed by the hydrothermal liquefaction (HTL) of the residue. In protein extraction, chemicals, buffers solution, and mechanical methods have been used. The residue after extraction was treated at the temperature of 270, 300, and 330 °C with the holding time of 30 min. Highest protein yield were from sonication followed by chemical extraction using Acetone and the extractive buffer solution. Bead mill extraction gave the lowest yield; however, it had a very good quality of protein and easy to scale-up so the residue of microalgae was produced from pilot-scale ball milling machine. After the HTL and hexane extraction, bio-crude can be obtained at 9%, 11%, and 16% of the total dried mass of microalgae residue for the 30-min residence time HTL at 270, 300, and 330 °C, respectively. Property of bio-crude was analyzed by gas chromatography with mass spectrometry. Results showed that the major components of bio-crude found in all HTL condition were Benzenes and Alkylbenzenes, Indole derivatives, and Pyridines, and Phenols.
Keywords:
microalgae, hydrothermal liquefaction, protein extraction, bead mill, sonication, bio-crude
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Pyrolysis and other biomass liquefaction technologies
Event:
25th European Biomass Conference and Exhibition
Session:
3AV.3.32
Pages:
1180 - 1185
ISBN:
978-88-89407-17-2
Paper DOI:
10.5071/25thEUBCE2017-3AV.3.32
Price:
FREE
