Title:
Alkaline Peroxide Oxidation Pretreatment of Corn Cob and Rice Husk for Bioconversion into Bio-Commodities: Part A- Enzymatic Convertibility of Pretreated Rice Husk to Reducing Sugar
Author(s):
Ayeni, A.O., Ogu, R., Awosusi, A.A., Daramola, M.O.
Document(s):
Paper
Poster
Abstract:
In this study, a 23-central composite design (CCD) of experiments was adopted using MINITAB 15 statistical software to investigate the effect of pretreatment conditions on enzymatic digestibility of rice husks to reducing sugar and establish the optimum pretreatment conditions for the process. Alkaline peroxide oxidation pretreatment conditions were chosen at low–high values of 60–90 oC reaction temperature, 6–10 h reaction time, and 1–3%(v/v) H2O2 concentration. The optimized conditions were established and validated at 100 oC, 4.6 h, and 0.3% (v/v) H2O2 to obtain sugar yield of 246 mg/g after 96 h. Variations of enzyme and substrate loadings at the optimized pretreated conditions established that sugar yield increased to about 271 mg/g for 30 g/L biomass loading at 25 FPU/g biomass after 96 h and temperature of 45 oC. Increasing enzyme loading beyond 25 FPU/g biomass did not result in an increase in sugar yield. Stereoscope microscopy, scanning electron microscopy (SEM-EDX) images, and FTIR spectroscopy reveal physical and chemical changes to the rice husk after pretreatment and enzyme conversion. The pretreatment methodology investigated is suitable for lignocellulosic biomass such as rice husks to produce reasonable quantity of reducing sugar required to produce bio-commodities like biofuel and fine chemicals.
Keywords:
biomass, cellulose, enzymatic hydrolysis, lignocellulose, rice husks
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Biorefineries
Event:
24th European Biomass Conference and Exhibition
Session:
3CV.2.71
Pages:
1225 - 1232
ISBN:
978-88-89407-165
Paper DOI:
10.5071/24thEUBCE2016-3CV.2.71
Price:
FREE
