Title:
Enzyme Synergism: A Powerful Tool for Decreasing Enzyme Loading for Efficient Biomass Conversion
Author(s):
Pletschke, B., Malgas, S., Bhattacharya, A., Bhattacharya-Shrivastava, A., Clarke, M.D., Mafa, M.S., Morake, S. , Thoresen, M.
Document(s):
Paper
Abstract:
Biomass conversion technologies for the production of fuels and value added products have rapidly gained support over the past few decades. The enzymatic conversion of lignocellulosic wastes has been particularly attractive, due to environmental and sustainability concerns. One of the major challenges in the production of cost effective biofuel (via the enzymatic hydrolysis route) is the cost associated with enzymes and enzyme loading. Our studies, and those of others, have conclusively shown that the enzyme loading can greatly be reduced by combining the enzymes in optimal ratios so as to improve the enzyme conversion efficiency. In this mini-review, we will highlight some of the major considerations that impact on enzyme synergism: enzyme selection, enzyme loading and ratios, sequential versus simultaneous and successive synergy, non-productive adsorption and product inhibition. Examples of enhanced hydrolysis of various substrates, ranging from simple defined model substrates to complex natural lignocellulosic substrates (feedstocks) are discussed. In each case, the enhanced production of sugars is clearly evident.
Keywords:
biomass, lignocellulose, cellulase, synergism, enzyme loading, hemicellulases
Topic:
Biomass Resources
Subtopic:
Biomass feedstock, residues and by-products
Event:
24th European Biomass Conference and Exhibition
Session:
1BO.5.4
Pages:
68 - 82
ISBN:
978-88-89407-165
Paper DOI:
10.5071/24thEUBCE2016-1BO.5.4
Price:
FREE
