Title:
Fuel Ethanol Production Using Xylose Assimilating Thermosensitive Saccharomyces Cerevisiae Isolated from Date Palm Juice in Bangladesh
Author(s):
Talukder, A.A., Islam, S., Alam, F., Siddiqa, A., Miah, R., Lertwattanasakul, N., Yamada, M.
Document(s):
Paper
Abstract:
Four thermosensitive yeasts were isolated from Khejurer rosh [an overnight natural fermented date palm (Phoenix dactylifera) juice/sap] at low temperature (~5-15 °C) for the production of bioethanol. Cultural, morphological, physiological, biochemical and genetical analysis were carried out under various physiological conditions. All 4-strains (Dj-1, Dj-2, Dj-3 and Dj-4) could produce bioethanol and their production rates were further investigated under various carbon sources, growth temperatures and pHs. Among them, the highest 10% (v/v) bioethanol was estimated from the yeast strain Dj-3, which was grown in the medium containing 18% of total sugars and 0.05% (NH4)2SO4 at optimum temperature and pH 25 °C and 6.0, respectively. Microscopic study and a partial 26S rDNA (D1/D2 region) sequencing results revealed that Dj-1, Dj-2, Dj-3 and Dj-4 strains encoded yeast strains were Saccharomyces cerevisiae, Pichia kudriavzevii, Saccharomyces cerevisiae and Debaryomyces nepalensis, respectively. The latter two strains Dj-3 and Dj-4 could grow well in the medium containing xylose as a carbon source. This is a first report in the world that the Saccharomyces cerevisiae (Dj-3) tolerates xylose. Our results conclude that the strain Dj-3 is a natural mutant strain of Saccharomyces cerevisiae, which would be an industrially potential candidate for bioethanol production.
Keywords:
bioethanol, xylose, yeast, khejurer rosh, fermented date palm juice
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Bioethanol and sugars from lignocellulosic biomass
Event:
24th European Biomass Conference and Exhibition
Session:
3BO.3.2
Pages:
1006 - 1012
ISBN:
978-88-89407-165
Paper DOI:
10.5071/24thEUBCE2016-3BO.3.2
Price:
FREE
