Title:
Hydrolysis of Corn Stover with Acremonium Cellulases: Analysis of the Residual Enzymes Activities through the Process and Effect of Additives to Reduce the Enzymes Unspecific Adsorption
Author(s):
De Bari, I., Liuzzi, F.
Document(s):
Paper
Poster
Abstract:
Sugars from biomass represent a versatile platform for the production of a number of bio-based products. Cellulases enzymes play an important role as they can hydrolyse biomass at mild conditions. Cellulases are mostly produced from Trichoderma and Aspergillus fungi. Although less investigated, another fungus, Acremonium cellulolyticus produces effective cellulases for the conversion of lignocellullosic substrates. The present paper describes the conversion of corn stover steam pretreated by Acremonium enzymes. The performaces of the Acremonium cellulases were compared with commercial Trichoderma and Aspergillus blends (Celluclast and Novozyme). The results indicated that, at the same process conditions, Acremonium cellulases were more effective and yielded 20% more sugars than the reference blends. Furthermore, the addition of external hemicellulases (Multifect xylanase) to the Acremonium complex produced a xylan hydrolysis 20% higher than that produced by the addition of the same amount to the reference mixtures. These findings indicated an higher synergistic action of the enzymatic components in the Acremonium complex with the commercial hemicellulases mixtures. In order to prolong the enzymes life time during the enzymatic hydrolysis with the Acremonium complex, three additives, namely Tween 80, Tween 20 and BSA, were tested to reduce the unspecific enzymes adsorption. The residual enzymatic activities in the hydrolysates were monitored during the hydrolysis and the results compared with control systems. The findings indicated that the use of Tween 80 and Tween 20 were effective in reducing the adsorption of the cellobiose hydrolases.
Keywords:
enzymatic hydrolysis, steam explosion
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Bioethanol and sugars from lignocellulosic biomass
Event:
23rd European Biomass Conference and Exhibition
Session:
3DV.2.49
Pages:
1332 - 1335
ISBN:
978-88-89407-516
Paper DOI:
10.5071/23rdEUBCE2015-3DV.2.49
Price:
FREE
