Title:
A Two-Step, Bacterial-Yeast Complementation for Lignocellulose Conversion to Bio-Ethanol
Author(s):
Tosato, V., Altun, B., Bez, C., Bruschi, C.V.
Document(s):
Paper
Slide presentation
Abstract:
Lignocellulosic biomasses, such as crops, wood, grass and food waste, are sustainable potential resources for bio-ethanol production. Out of the three main steps needed for the conversion process (pretreatment, enzymatic degradation and fermentation), the pretreatment, which can be chemical or microbial, is the most crucial one. In this work we provide evidence that wood flour, a common waste sub-product of wood furniture manufacturing, is the ideal substrate for an efficient microbial pretreatment of lignocellulose. We isolated from the bovine rumen two strains of B. pumilus and B. licheniformis that were deeply characterized and tested for their ability in lignocellulose pretreatment. Moreover, the enzymatic degradation step can be partially completed by the same microorganisms producing a mixture of glucose and cellobiose. We further developed genetically engineered, recombinant yeast strain capable of vigorous growth using cellobiose as the only carbon source. We therefore demonstrated that the sequential treatment of wood flour firstly by Bacilli and secondly by S. cerevisiae allows an efficient and economic conversion of biomass into glucose leading to interesting perspectives in bio-ethanol production.
Keywords:
bioethanol, genetic improvement, lignocellulose, waste, yeast, bacteria
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Bioethanol and sugars from lignocellulosic biomass
Event:
23rd European Biomass Conference and Exhibition
Session:
3CO.7.3
Pages:
979 - 982
ISBN:
978-88-89407-516
Paper DOI:
10.5071/23rdEUBCE2015-3CO.7.3
Price:
FREE
