Title:
Enzymatic Conversion of Sucrose into Gluconic Acid by a Fed-Batch Process Operated in the Constant Feeding Mode
Author(s):
Taraboulsi, F.A.J., Yoriyaz, E.J., Vitolo, M.
Document(s):
Paper
Poster
Abstract:
The biomass bio-refinery is a facility in which huge sugarcane crops as well as other agricultural raw materials are transformed into several types of commercial products through different integrated processes. This work is based on the use of invertase (KM = 40.2mM; Vmax = 0.064UI/mL) and glucose oxidase (KM = 15.6mM; Vmax = 3.41x10-3UGO/mL), respectively, for hydrolyzing sucrose into glucose and fructose (inverted syrup containing both sugars at 1:1 ratio) and for oxidizing glucose into gluconic acid. Catalase (KM = 1.1µM; Vmax = 1.2 Ucat/mL) was also used as an auxiliary enzyme. Both reactions were evaluated through a fed-batch process whose sucrose (64 g/L) or glucose (32 g/L) solution was added according to a constant feeding mode [f = (Vf – Vo) ÷.; f = feeding rate (L/h), Vf = final volume (L), Vo = initial volume (L) and . = filling-up time (h)]. The invariable assay conditions were: pH = 5.0, agitation = 100 rpm, 37oC, Vo = 0.1L and Vf = 0.4L, whereas . was 1h and 4h for sucrose hydrolysis and glucose oxidation, respectively. The filling-up time (.) was set at 4h when the sucrose conversion into fructose and gluconic acid was carried out by the simultaneous action of invertase (780UI), glucose oxidase (7,000UGO) and catalase (230,000Ucat). Samples were taken at each 0.1h for analytical purposes. The yield of sucrose hydrolysis by invertase was 91%, whereas the yield of glucose oxidation by glucose oxidase was 60%. Nevertheless, the yield of the multienzymatic sucrose/(gluconic acid + fructose) conversion was 75%.
Keywords:
biotechnology, catalytic conversion, enzymatic process, enzyme
Topic:
Biomass Conversion Technologies for Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Biorefineries and chemicals production
Event:
23rd European Biomass Conference and Exhibition
Session:
3AV.3.9
Pages:
1054 - 1057
ISBN:
978-88-89407-516
Paper DOI:
10.5071/23rdEUBCE2015-3AV.3.9
Price:
FREE
