Title:
Validation of a High through put Analysis of Microalgae Carbohydrates for Bio-ethanol Production
Author(s):
Funcia, I., Alegria, I., Iruretagoyena, F.J., Munarriz, M., Piera, M., Reyes-Sosa, F.M., Echeverría, I.
Document(s):
Paper
Abstract:
The focus of this study was to validate a methodology for evaluating the potential of using microalgae for bio-ethanol production. For this work, samples of Red algae were fully characterized: carbohydrates, soluble sugars, lipids, ashes, moisture and protein content. Then, microalgae were subject to a two-step high-throughput micro-plate enzymatic hydrolysis process (1 ml working volume). Likewise, the assay was carried out with a higher working volume (up to 100ml) in erlenmeyer flasks for validating the analysis. In both cases, saccharification yields were measured by HPLC-RID technique quantifying sugars: changes on aqueous medium soluble sugars profile were analyzed during these stages to obtain the maximum glucose yield. Finally, fermentation trials were carried out in order to identify any toxic or inhibitory effect over standard ethanologenic yeast, Saccharomyces cerevisiae. The development of this high through put analysis methodology has the following advantages: -Reduce the amount of raw material, which is normally a limiting factor -Reduce working time and costs because allows to carry out different trials at the same time -Allow comparing among different parameters conditions (for example enzymes dosage, solid: liquid ratio…) -Flexible methodology that can be optimized for other microalgae species
Keywords:
enzymatic hydrolysis, fermentation, microalgae
Topic:
Biomass resources
Subtopic:
Research on energy crops (session 3)
Event:
18th European Biomass Conference and Exhibition
Session:
VP1.3.36
Pages:
499 - 502
ISBN-13:
978-88-89407-56-1
ISBN-10:
88-89407-56-5
Paper DOI:
10.5071/18thEUBCE2010-VP1.3.36
Price:
FREE
