Pretreatment and Fractionation of Wheat Straw by Organosolv in Biphasic System
Viola, E., Morgana, M., Zimbardi, F., Cerone, N., Romanelli, A., Valerio, V.
In this work we investigated the pretreatment of wheat straw in a biphasic system constituted by an organic solvent (butanol) and oxalic acid solution. The pretreatments were carried out in a 300 mL Parr reactor at total solid load of 5 wt%; temperature 140-180 °C, time 30-90 min and load of oxalic acid 0-10%. The pretreated straw was fractionated in the main constituents: cellulose, hemicellulose and lignin. The cellulose fraction has undergone enzymatic hydrolysis tests with commercial mix of enzymes in shaken flasks at 50°C up to 72h. The Organosolv treatment was optimized to maximize the purity of the cellulose and hemicellulose fractions and the glucose yields after enzymatic hydrolysis, by using an experimental design and response surface methodology. The results showed that the treatment is highly efficient to fractionate and deconstruct the straw, since its conversion into glucose, by enzymatic hydrolysis, reached the yield of 98%.
straw, pretreatment, second generation, hydrolysis, biobased economy
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