Title:
Hydrothermal Pretreatment of Lignocellulosic Biomass and Model Compound and their Effect on Enzymatic Hydrolysis
Author(s):
Syaftika, N., Matsumura, Y.
Document(s):
Paper
Abstract:
We herein report the behavior of three major lignocellulosic biomass components namely lignin, hemicellulose and cellulose under hydrothermal pretreatment and its effect on enzymatic hydrolysis for glucose production. The aim is to investigate the effect of structural cross-link between the three major components by comparing the actual biomass where the components are connected to each other, with model compounds where each compounds are separated. An actual biomass was chosen and the model compound of isolated cellulose, xylan and lignin were used according to the actual biomass lignocellulose composition. To achieve the goal, the autoclave reactor was used to conduct hydrothermal pretreatment in a temperature range 150 - 250 °C for 30 minutes. To evaluate the effectiveness of the pretreatment, an enzymatic hydrolysis was done subsequently using cellulase and ß-glucosidase at temperature 40 °C for 48 h and the glucose product was measured by HPLC. The remaining amount of cellulose was measured after the enzymatic hydrolysis. The comparison between the behavior of cellulose and the efficiency of enzymatic hydrolysis under the influence of lignin and hemicellulose presence in actual biomass and model compound was clarified in this study.
Keywords:
cellulose, rice straw, hydrothermal pretreatment, glucose
Topic:
Biomass Conversion Technologies for Fuels, Chemicals and Materials
Subtopic:
Bio-alcohols from lignocellulosic biomass and pretreatment
Event:
26th European Biomass Conference and Exhibition
Session:
3DV.4.21
Pages:
1183 - 1188
ISBN:
978-88-89407-18-9
Paper DOI:
10.5071/26thEUBCE2018-3DV.4.21
Price:
FREE
