Title:
Improving In-Situ Lipid Extraction Efficiency from Oleaginous Yeast in a Lipid Extractor for Biofuel Application
Author(s):
Chopra, J., Sen, R.
Document(s):
Paper
Poster
Abstract:
Biofuel production using oleaginous yeasts hasimminent potential to serve as a promising alternativeto other popularly used renewable feedstockssuch as lignocellulosic and microalgal biomass. Their superior properties includehigher growth rates, cultivation of them being independent of climatic condition and zero land requirements. In the current work, the batch- biomass cultivation method at nitrogen limiting condition (1.23 g L-1 ammonium chloride) led to maximum biomass and lipid yield (biomass concentration of 20 g L-1 with a lipid content of 57 ± 1.2 % w/w, DCW basis) from the oleaginous yeast Meyerozyma caribbica. Subsequently, the lipid extraction efficiency from the biomass was compared using various methods, in which, the biomass was first subjected to various cell disruption methods, followed by lipid extraction with solvents. The results of the same were compared againstthe energy efficient “in-situextraction” method. We also found that the direct extraction of the neutral lipid fraction from the wet-yeast biomass in a soxhlet-type lipid extractor was equally competent to other tested extraction procedures. Through soxhlet extraction procedure, using Methanol-Hexane solvent system, neutral lipids of about 40 ± 1.3 % w/w of DCWcould be extracted from Meyerozyma caribbica biomass. Further studies to understand the effect of critical parameters influencing the lipid extraction namely, biomass loading, polarity of solvent system, cycle time (boiling, rinsing and recovery) and moisture contentare currently being investigated to improve the performance of soxhlet-extraction for neutral lipid using Taguchi design of experiments. Thus, the developed in-situ neutral lipid extraction method involves lesser steps with improved product yield and is energetically efficient than the multi-step lipid extraction process.
Keywords:
biodiesel, lipids, yeast, extraction, in situ
Topic:
Biomass Conversion Technologies for Fuels, Chemicals and Materials
Subtopic:
Production and application of biobased chemicals
Event:
26th European Biomass Conference and Exhibition
Session:
3AV.5.3
Pages:
1066 - 1070
ISBN:
978-88-89407-18-9
Paper DOI:
10.5071/26thEUBCE2018-3AV.5.3
Price:
FREE
